Figure 3

Elp3 is required for cranial neural crest (CNC) migration in explants and transplantation experiments.

(a) CNC explants were dissected from early neurula embryos, plated on FN (10 μg/ml) and imaged at 0, 5, 12 and 20 h. The surface areas of each explant at every image point were measured and the ratios of the areas to the values at 0 hour are shown by the line plot (b). The data were represented as the means ± standard deviation (SD). **P < 0.01; ***P < 0.001, two-tailed T-test. (c) GFP mRNA (100 pg) was injected into one cell at the 4-cell stage with or without Elp3 morpholino (25 ng). At St.15–16, GFP-labeled CNC explants were dissected and grafted into normal host embryos. The status of the fluorescent neural crest was imaged at the tailbud stage (St.35).