Figure 4: Monoclonal flow analysis of clones randomly selected from the final round of cell-based biopanning against each of the membrane bound antigens, for binding to CHO cells transfected with the respective GFP-tagged antigen.
From: Targeting membrane proteins for antibody discovery using phage display
Clones were analyzed by the ratio of the percentage of cells showing both GFP expression and phage binding (Q2 quadrant) to the percentage of cells showing phage binding to non-GFP expressing cells (Q1 quadrant). (a) Phage clones from CD83-GFP biopanning; (b) Phage clones from CD117-GFP biopanning; (c) Phage clones from CD11b-GFP biopanning. Non-specific clones binding to an unknown irrelevant antigen on the CHO cell surface are highlighted in red. (d) Scattergrams showing example clones taken from the CD117-GFP panning that show specific binding (Specific Clone G1), non-specific binding (Non-Specific Clone F4) and no-binding (Non-Binding Clone F5).
