Figure 5: Flow cytometry test for specificity of clones isolated by cell-based biopanning on GFP-fused target antigens.
From: Targeting membrane proteins for antibody discovery using phage display

CHO cells were transfected with CD83-GFP (a–c), CD117-GFP (d–f) or CD11b-GFP (g–i) and then probed with either Clone C2 isolated by biopanning of CD83-GFP (a,d,g), Clone A3 isolated by biopanning on CD117-GFP (b,e,h) or Clone H7 isolated by biopanning on CD11b-GFP (c,f,i).