Figure 6: Testing of phage-derived, reformatted antibodies on native sources of their respective antigens.
From: Targeting membrane proteins for antibody discovery using phage display
(a) Anti-CD83 mAbs were tested for binding to KM-H2 cells by flow cytometry. 3C12, isolated by biopanning against recombinant soluble CD83, is shown in blue. 3C12C is the affinity matured version of 3C12 (purple) and C2 was isolated by cell-based biopanning against CD83-GFP (teal). The control samples were cells only (shaded) and cells treated with secondary antibody only (red). The median fluorescence is indicated above the test samples. (b) Anti-CD117 mAbs were tested for binding to canine mast cell tumour tissue sections by immunohistochemistry. Sections were probed with secondary antibodies only (left), positive control anti-human c-Kit (middle) and A3 mAb isolated by cell-based biopanning on CD117-GFP (right). Scale bar represents 50 μm.
