Figure 1: Nrg4 gene expression was reduced in liver and EWAT of obese mice.

Eight-week-old male C57BL/6 mice were fed a chow or HFD for total 12 weeks, Total RNA was extracted and relative mRNA levels of Nrg4 was determined in liver (a), EWAT (b), SubWAT (c), and BAT (d) by real-time PCR. *P < 0.05 compared to chow-fed mice (n = 3).