Figure 6: NRG4 improved insulin resistance in obese mice.

C57BL/6 obese mice fed an HFD were hydrodynamically injected via tail vein with 20 μg of pLIVE-NRG4 or pLIVE-SEAP plasmid for 3 weeks. (a) Change of body weight; (b) Fat and lean mass measured 3 weeks after gene transfer; (c) Total food intake; (d) Time-dependent blood glucose level in glucose tolerance test (1.5 g/kg); (e) Area under the curve from glucose tolerance test; (f) Relative ratio of glucose concentration in insulin tolerance test (0.75 U/kg). *P < 0.05, **P < 0.01 compared to control animals (n = 5).