Figure 2: HPLC chromatograms of synthesized oligosaccharides.
From: Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate

Both DEAE-HPLC and PAMN-HPLC methods were used to analyze the purity of each individual oligosaccharide. Overall, the purity for each compound was determined to be >90%.