Figure 4: The minimal HS binding motif of HS20 was a 6mer with both 2-O-sulfation and 6-O-sulfation. | Scientific Reports

Figure 4: The minimal HS binding motif of HS20 was a 6mer with both 2-O-sulfation and 6-O-sulfation.

From: Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate

Figure 4

(a) Structures of the synthetic oligosaccharides (4mer to 12mer) with both 2-O and 6-O-sulfation. (b) The 2-O and 6-O sulfated oligosaccharides with lengths longer than 4mer inhibited HS20 binding. Competitive ELISA was performed by pre-incubating oligosaccharides with HS20 for 30 minutes, and then adding the mixture into an ELISA plate to detect the binding to GPC3.

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