Figure 5: The addition of a 3-O-sulfation to oligosaccharides further inhibits HS20 binding.
From: Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate
(a) Structures of the synthetic oligosaccharides (4mer to 12mer) with both 2-O and 6-O-sulfations (mer-5), with an extra 3-O-sulfation (mer-6), and with two extra 3-O-sulfations (mer-7). (b) The oligosaccharides with an extra 3-O-sulfation showed more inhibition of HS20 binding. Competitive ELISAs were performed on various 4mer, 6mer and 12mer oligosaccharides. Dashed lines indicate the value of IC50. (c) A competitive ELISA with various 12mers that have differences in the sulfation position and number.
