Figure 7: A comparison of Wnt and HS20 binding motifs on oligosaccharides.
From: Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate
(a) Hypothetical schematic structures for HS20-binding HS. HS20-binding oligosaccharides require both 2-O and 6-O sulfations (bold). The site of 3-O-sulfation (bold) is shown by a green box. The minimal oligosaccharide recognized by HS20 is shown as a 6mer (light blue region) or as a 4mer with a 3-O-sulfation (purple region). (b) Hypothetical schematic structures for Wnt-binding HS. Wnt-recognized oligosaccharides require 6-O sulfation (bold). The sites of 3-O-sulfation are shown by green boxes. The minimal oligosaccharide recognized by Wnt is an 8mer (light pink region) or a 6mer with a 3-O-sulfation (orange region).
