Table 1 Summary of ESI-MS analysis of synthesized oligosaccharides.
From: Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate
Oligosaccharides | Abbreviated structures | Calculated MW | Measured MW |
|---|---|---|---|
12mer-1 | GlcNAc-GlcA-GlcNAc-GlcA-GlcNAc-GlcA-GlcNAc-GlcA-GlcNAc-GlcA-GlcNAc-GlcA-pNP | 2415.0 | 2415.6 ± 1.0 |
12mer-2 | GlcNS-GlcA-GlcNS-GlcA-GlcNS-GlcA-GlcNS-GlcA-GlcNS-GlcA-GlcNS-GlcA-pNP | 2643.1 | 2642.8 ± 0.7 |
12mer-3 | GlcNS6S-GlcA-GlcNS6S-GlcA-GlcNS6S-GlcA-GlcNS6S-GlcA-GlcNS6S-GlcA-GlcNS6S-GlcA-pNP | 3123.5 | 3122.8 ± 0.2 |
12mer-4 | GlcNS-GlcA-GlcNS-IdoA2S-GlcNS-IdoA2S-GlcNS-IdoA2S-GlcNS-IdoA2S-GlcNS-GlcA-pNP | 2963.4 | 2963.3 ± 0.7 |
12mer-5 | GlcNS6S-GlcA-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 3443.7 | 3442.9 ± 0.4 |
12mer-6 | GlcNS6S-GlcA-GlcNS3S6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 3523.8 | 3524.7 ± 2.2 |
12mer-7 | GlcNS6S-GlcA-GlcNS3S6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS3S6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 3603.8 | 3603.1 ± 1.5 |
10mer-5 | GlcNS6S-GlcA-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 2866.2 | 2866.2 ± 0.9 |
8mer-5 | GlcNS6S-GlcA-GlcNS6S-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 2288.8 | 2289.3 ± 0.4 |
6mer-5 | GlcNS6S-GlcA-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 1711.4 | 1710.9 ± 0.5 |
4mer-5 | GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP | 1214.0 | 1214.0 ± 0.6 |
4mer-6 | GlcNS3S6S-IdoA2S-GlcNS6S-GlcA-pNP | 1294.0 | 1293.8 ± 0.3 |
