Table 5 Primer pairs used in qRT-PCR analysis.
From: Tantalum implanted entangled porous titanium promotes surface osseointegration and bone ingrowth
Gene | Primers (F = forward, R = reverse) | Amplicon |
|---|---|---|
Col1a1 | F: ACCTCCGGCTCCTGCTCCTCTTAG | 235 |
R: GCGCCGGGGCAGTTCTTGGTCT | ||
ALP | F: GACAATCGGAATGAGCCCACAC | 222 |
R: GTACTTATCCCGCGCCTTCACCAC | ||
OPN | F: TCTGATGAATCTGATGAACTGGTC | 195 |
R: GGTGATGTCCTCGTCTGTAGCA | ||
BSP | F: AAGAAGGGGAAGAAGAAAGTGTCA | 218 |
R: GTATTCATTGGCGCCCGTGTATTC | ||
OC | F: AGCCCAGCGGTGCAGAGTCCA | 224 |
R: GCCGTAGAAGCGCCGATAGG | ||
OSX | F: GCTGCCCACCTACCCATCTGACTT | 207 |
R: CTGCCCCCATATCCACCACTACCC | ||
β-actin | F: CCCAAGGCCAACCGCGAGAAGATG | 219 |
R: GTCCCGGCCAGCCAGGTCCAGA |
