Figure 4
From: Development of antithrombotic nanoconjugate blocking integrin α2β1-collagen interactions
(A) Schematic diagram of examination on the inhibition efficiency of L-PGMA NPs in platelet adhesion in vitro. (B) The integral enthalpy profile of free LWWNSYY dissolved in normal saline titrated into collagen solution (#1), free LWWNSYY dissolved in normal saline containing 25% (v/v) ethylene glycol (EG) titrated into collagen solution (#2) and collagen titrated into L-PGMA NPs dissolved in normal saline (#3). The fit to the single-site binding model is shown by continuous curve. (C) Dose-response inhibition ratio of free LWWNSYY (red) and L-PGMA NPs (blue) on the platelet adhesion on collagen peptide. Continuous curves are the best fit obtained with the Hill equation. The obtained maximum inhibition ratio and the concentration required to inhibit half of the specific binding of inhibitors are shown in the inset. Data was presented as the mean ± S.D. from eight independent experiments (N = 8).
