Figure 2

Poly(I:C) inhibits melanoma lung metastasis, Chi3l1 and IL-13Rα2 via a MAVS-dependent and TLR3-independent mechanism(s).

(a,b) WT, TLR3 null (TLR3^−/−) or MAVS null (MAVS^−/−) mice were given B16 melanoma cells (+) or vehicle (−) and treated with Poly(I:C) (+) or control vehicle (−). Lung metastasis was evaluated 2 weeks later. Representative lungs and quantification of pleural melanoma counts in WT (+/+) and TLR3 null mice (−/−). (c,d) Representative lungs and pleural melanoma counts in WT (+/+) and MAVS null mice (−/−). (e,f ) Quantification of BAL Chi3l1 and whole lung IL-3Rα2 mRNA in WT mice (+/+) and MAVS null (−/−) mice challenged with B16 cells (+) or vehicle control (−). Panels a and c are representative of a minimum of 5 mice in each group. The values in panels b,d–f represent the mean ± SEM of evaluations with a minimum of 4 mice. **P < 0.01. ns, not significant.