Figure 5

RLH activation enhances NK cell accumulation and activation in melanoma challenged lungs.

(a,b) WT (+/+) or MAVS null mice (−/−) were given B16 melanoma cells or their vehicle and treated with Poly(I:C) or vehicle control. NK cells were evaluated 2 weeks later. Proportion of NK1.1⁺/CD3⁻ NK lymphocytes and ratio to myeloid derived suppressor cells (MDSC) in lung lysates were evaluated by FACS analysis. (c–e) mRNA expression of NK cell activating receptor natural killer group 2D (NKG2D) and cytolytic enzymes granzyme B (GRZNB) and perforin in the lungs were evaluated by qRT-PCR. The values in the panels represent the mean ± SEM of evaluations in a minimum of 4 mice. *P < 0.05. **P < 0.01. ns, not significant.