Figure 2: STMV progeny analysis.

(A) STMV plus (+) and minus (−) strand progeny RNA and CP were respectively probed by Northern and Western blot analysis. RT-PCR assays of wt STMV and the indicated CP variants were performed using total RNA isolated from leaves harvested at 4 dpi. (B) Quantitative analysis of (+) and (−) progeny RNA of wt STMV and each CP variant was performed by scanning the autoradiographs using a Typhoon Phosphoimage scanner (Model 9410, GE Health Care) followed by ImageQ software analysis. Data was quantitated and compared internally with each levels of (+) and (−)-strands to that of wild type. Conditions for Northern and Western blots and RT-PCR analysis are as described previously⁹.The positions of full length and truncated CP, full length and truncated STMV RNA are indicated on the left. (C) Virion analysis⁹. Electron microscopic images of negatively stained (with uranyl acetate) purified virions of wt STMV, CPKO, CP 13aa and CPΔ13aa are shown. Bar = 50 nm.