Figure 5
From: Decellularization of human stromal refractive lenticules for corneal tissue engineering
Recellularization on SDS-decellularized lenticules.
(A) Confocal immunofluorescence showed similar expression of paxillin (focal adhesion complex protein) and phalloidin (F-actin fibers) in human stromal fibroblasts cultured on SDS-treated lenticules and collagen I-coated glass surface for 72 hours. Scale bars: 10 μm. (B) TEM micrographs showing SDS-treated lenticules before cell seeding and after culture with stromal fibroblasts for 2 weeks. Cells were closely adhered on the lenticule surface and did not show any signs of cellular toxicity, including dilated endoplasmic reticulum (ER) and induction of lysosomes and intracellular vesicles. Scale bar: 0.5 μm.
