Table 2 Decellularization conditions.
From: Decellularization of human stromal refractive lenticules for corneal tissue engineering
Treatment | Protocol with continuous agitation | |
|---|---|---|
1 | Control | In PBS |
2 | Hyperosmotic | (i) 1.5 M NaCl in PBS (24 h) * # § ς |
3 | Ionic detergent | (i) 0.1% SDS in PBS (24 h) *# § |
4 | Non-ionic detergent | (i) 0.1% Tx in PBS (24 h) # |
5 | Ionic & non-ionic detergents | (i) 0.1% SDS |
(ii) 0.1% Tx in PBS (24 h) Φ | ||
6 | Enzymatic (2Nu) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 2 U/ml nuclease in NaCl/PBS (24 h, twice) | ||
7 | Enzymatic & detergent (2Nu + SDS) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 2 U/ml nuclease in NaCl/PBS (24 h, twice), | ||
(iii) 0.1% SDS in PBS (24 h) | ||
8 | Enzymatic (5Nu) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 5 U/ml nuclease in NaCl/PBS (24 h, twice) Φ ς | ||
9 | Enzymatic & detergent (5Nu + SDS) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 5 U/ml nuclease in NaCl/PBS (24 h, twice), | ||
(iii) 0.1% SDS in PBS (24 h) | ||
10 | Enzymatic (10Nu) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 10 U/ml nuclease in NaCl/PBS (24 h, twice), | ||
11 | Enzymatic & detergent (10Nu + SDS) | (i) 1.5 M NaCl in PBS (24 h), |
(ii) 10 U/ml nuclease in NaCl/PBS (24 h, twice), | ||
(iii) 0.1% SDS in PBS (24 h) |
