Figure 1: Detection of β-glucan products in vitro. | Scientific Reports

Figure 1: Detection of β-glucan products in vitro.

From: Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers

Figure 1

(A) Laminaria hydrolysis using a mixture of endo-β-1,3-glucanase and exo-β-1,3-glucanase and the quantity of released glucose determined via the anthrone-H2SO4 method and verified via GC/MS; error bars indicate SD (n = 3). (B) β-1,4-Glucan products synthesized in vitro after endo-β-1,3-glucanase and exo-β-1,3-glucanase hydrolysis to remove callose: the total glucose released by the β-1,4-glucanases was defined as non-crystalline cellulose, and the remaining pellet was defined as crystalline cellulose; All experiments were conducted in biological triplicate and data are presented as the mean ± SD (n = 3). (C) β-1,4-Glucan and β-1,3-glucan products synthesized using different centrifugation speeds for the isolation of detergent-soluble membrane proteins; error bars indicate SD (n = 3).

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