Figure 2: Identification of GhCESA1 and GhCESA8 proteins and detection of β-1,4-glucan and β-1,3-glucan products in vitro from total membrane extracts of cotton fibers treated with/without cellulase. | Scientific Reports

Figure 2: Identification of GhCESA1 and GhCESA8 proteins and detection of β-1,4-glucan and β-1,3-glucan products in vitro from total membrane extracts of cotton fibers treated with/without cellulase.

From: Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers

Figure 2

(A) Western blot analysis of GhCESA1 and GhCESA8 (upper lane), separation of total membrane proteins by SDS-PAGE and staining with Coomassie Blue (middle lane) and relative levels of both proteins (lower lane). The relative protein level was measured by calculating the protein band area x protein band intensity. (B) Products of β-1,4-glucans (upper lane) and β-1,3-glucans (lower lane) synthesized in vitro from cotton fiber extracts treated with different concentrations of cellulases; the data are presented as the mean ± SD (n = 3).

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