Table 1 β-1,4-glucan and β-1,3-glucan products synthesized in vitro using different ultracentrifugation methods for the extraction of membrane proteins from cotton fibers.

From: Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers

Ultracentrifugation method

Glucan

Counts per minute (cpm) incorporated (cpm mg −1 protein)

SE/SU

Total nmol of glucose incorporated (nmol mg −1 protein)

SE/SU

Sucrose density (SU)

β-1,4-glucan

5,670 ± 190

 

50.0 ± 2.0

 

β-1,3-glucan

12,900 ± 1,000

 

113 ± 9.0

 

Sedimentation velocity (SE)

β-1,4-glucan

10,100 ± 400

1.78

89.0 ± 3.0

1.78

β-1,3-glucan

22,200 ± 1,500

1.72

195 ± 14

1.73

  1. The data presented as mean ± SD (n = 3).
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