Table 4 Classification of the novel proteins associated with cellulose and callose synthase complexes in cotton fibers.

From: Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers

Group

Protein name*

Total peptides (Exp 1)

Total peptides (Exp 2)

Total peptides (Exp 3)

Accession number

TM domain

Protein fuction

Blank (B)

Treated (T)

Blank (B)

Treated (T)

Blank (B)

Treated (T)

CESA-associated

I

Phenylcoumaran benzylic ether reductase-like protein

3(2) @

8(5)

2(1)

3(2)

3(3)

6(5)

gi|124488476

0

isoflavone reductase

vacuolar H+ -ATPase catalytic subunit

8(4)

16(9)

0(0)

4(3)

11(9)

15(8)

gi|167313

0

cytoskeleton, ATP binding, hydrolase activity

II

glyceraldehyde-3-phosphate dehydrogenase C subunit

48(18)

44(18)

37(12)

17(8)

16(12)

16(12)

gi|211906518

0

NAD binding, NADP binding, oxidoreductase activity

fasciclin-like arabinogalactan protein

2(1)

2(2)

2(1)

7(4)

5(4)

5(5)

gi|606942

1

cell wall biogenesis, acetyl-CoA metabolic process

UDP-glucuronic acid decarboxylase 2

15(7)

20(8)

8(5)

0(0)

10(6)

21(11)

gi|213950353

1

UDP-xylose metabolic, UDP-glucuronate decarboxylase activity

tonoplast intrinsic protein

0(0)

1(1)

2(1)

2(1)

1(1)

1(1)

gi|227434194

6

water channel, facilitate the transport of urea and hydrogen peroxide

CALS-associated

I

ubiquitin extension protein

8(5)

12(5)

5(3)

7(3)

9(4)

10(4)

gi|73761683

0

structural constituent of ribosome, ubiquitin-dependent protein catabolic process

3-ketoacyl-CoA reductase 1

5(3)

19(10)

1(1)

5(3)

6(3)

8(5)

gi|62956018

1

acetoacetyl-CoA reductase, ketoreductase, oxidoreductase

II

plasma membrane H+ -ATPase, partial

2(1)

3(2)

0(0)

2(1)

2(2)

2(2)

gi|2911803

9

ATPase activity, cation transport

  1. TM: Transmembrane; I: Proteins showing more total peptide counts and distinct peptide numbers upon cellulase treatment; II: Proteins without more detectable peptides upon cellulase treatment.
  2. *Based on bioinformatic analysis of Arabidopsis homologs of the 19 cotton fiber proteins identified via LC-MS/MS (Supplementary Table S7); (B)/(T): Treated without/with 1% cellulose.
  3. @Indicated total peptide counts and distinct peptide numbers (brackets).