Figure 3
From: Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice
Flow cytometric analysis of brain infiltrating leukocytes during tumor progression.
(a). CD45+/low leukocytes extracted from the brain before (D0), or 21 (T-D21) or 28 (T-D28) days after tumor grafting were analyzed for the expression of CD11b and CD45 and subdivided into CD45+CD11b− (denoted as I), CD45+CD11b+ (denoted as II) and CD45 lowCD11b+ (denoted as III) populations. The percentage of cells in each population is indicated. (b). Histogram depicting the number of brain infiltrating cells corresponding to pooled populations I and II (see a) in control (D0), sham-operated (S-D21 and S-D28) or and tumor implanted (T-D21 and T-D28) mice. Pooled data from individual mice are represented as mean ± SEM (D0: n = 5, S-D21: n = 7, S-D28: n = 5, T-D21: n = 12 and T-D28: n = 13). Statistical significance was evaluated by a Mann Whitney test: ***p < 0.001, ns: non significant. (c) Relative representation of populations I, II and III (see a) in brain in the different groups of mice. Data are presented as the mean ± SEM in each group. (d) Percentages of LysM-EGFP+ (green), CD11c-EYFP+ (orange) and LysM-EGFP+/CD11c-EYFP+ (blue) cells among populations I, II and III (see a) found in the brain of sham-operated (S-D21 and S-D28) or tumor implanted (T-D21 and T-D28) mice. The surface of the pie charts is proportional to the absolute cell number in the specified population. Cell numbers are indicated under each pie chart as mean ± SEM (S-D21: n = 4, S-D28: n = 4, T-D21: n = 6 and T-D28: n = 6).
