Figure 4

Multiparametric analysis of brain infiltrating cells allowed identification of all infiltrating leukocytes.

(a) Absolute numbers of lymphocytes (T: T cell, B: B cell and NK: NK cell), granulocytes (Ne: Neutrophils and Eo: eosinophils), DCs (P: pDC, Xc:XCR1⁺DC and Cd: CD11b⁺DC) and monocytes/macrophages (Mi: microglia and P1, P2, P3, P4, P5 cells as defined in¹⁵ in the brain of sham-operated (S-D21 and S-D28 are grouped) and tumor-implanted (T-D21; T-D28) mice at D21 and D28. Data are presented as mean ± SEM (n = 12). Multiple comparisons were made using one-way ANOVA. Statistically significant differences are observed between T-D28 and S-D21/D28 or T-D21 with a ***p < 0.001 for most of the populations with the exception of B cells, neutrophils, eosinophils, P4, P5 and microglia. No statistically significant differences are observed between S-D21/28 and T-D21. (b) Frequency distribution and differentiation chart of the precursor P1 population, the mo-DC population (P2–P3) and the Macroph. population (P4-P5) at D21 and D28. mo-DC: monocyte-derived DCs; Macroph.: Macrophages. *p < 0.01 P2/3 vs. P4/5; ^$p < 0.01 D21 vs. D28, Mann-Whitney test.