Figure 5
From: The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice
Map-based cloning of the lc7 gene.
(A) The lc7 gene was mapped to a 78-kb region between the markers S16 and S22 on the long arm of chromosome 7 with nine candidate genes. The LOC_Os07g46460 gene encoding Fd-GOGAT1 in the lc7 mutant with a single-base substitution at the 983rd position is the best candidate gene of lc7. (B,C) PCR amplification of Hyg and LC7 from the control and transgenic plants. M = Trans2k plus DNA Marker (http://www.transgen.com.cn). (D,E) The relative expression level of Fd-GOGAT1 in transgenic plants. (F) The expression patterns of Fd-GOGAT1 in different tissues as analyzed by qRT-PCR. (G) The Fd-GOGAT activity in wild-type, lc7 and complemented lc7 plants. (H) The phenotypes of T1 transgenic plants and their parents at the booting stage. (I) The lesion length of lc7 mutant and complemented lc7 plants 14 days after inoculation with Xoo strain PXO99. Nip-V and lc7-V are transgenic plants from the Nipponbare and lc7 mutant transformed with the pCAMBIA1301 vector, respectively; lc7-C is the function-complemented lc7 plant; and NRi is the OsFd-GOGAT1 RNAi plant. The scale bar is indicated.
