Figure 3: The surface expression of ANO1 was enhanced by 14-3-3γ. | Scientific Reports

Figure 3: The surface expression of ANO1 was enhanced by 14-3-3γ.

From: Suppression of 14-3-3γ-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells

Figure 3

(a) Cell surface biotinylation results from membrane protein fractions from HEK293T cells transfected with GFP-ANO1, FLAG-14-3-3γ, and/or HA-R18. Western blot images were cropped for clarification. Full blot images were included in supplementary information. (b) Bar graph showing densitometric quantification of (a). Data are expressed as mean ± SEM; *P < 0.05, **P < 0.01. (c) Representative traces of whole-cell recordings of COS7 cells transfected with GFP-ANO1, 14-3-3γ, and/or HA-R18. (d) Summary bar graph showing normalized current density as in (c) at +80 mV. Data are expressed as mean ± SEM; *P < 0.05.

Back to article page