Figure 5: Endogenous ANO1 associates with 14-3-3γ in U251 cells. | Scientific Reports

Figure 5: Endogenous ANO1 associates with 14-3-3γ in U251 cells.

From: Suppression of 14-3-3γ-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells

Figure 5

(a) Representative traces of whole-cell recordings of ANO1 in U251 cells transfected with Sc shRNA, ANO1 shRNA-1, and/or a GFP-ANO1-insensitive form of ANO1. (left panel). Summary bar graph showing normalized current density as in (left panel) at +80 mV (right panel). Data are expressed as mean ± SEM; ***P < 0.001. (b) Representative traces of whole-cell recordings of ANO1 in U251 cells treated with DMSO or A01 (an ANO1 inhibitor, 10 μM) (left panel). Data are expressed as mean ± SEM; **P < 0.01. Summary bar graph showing normalized current density as in (left panel) at +80 mV (right panel). (c) Representative immunocytochemical images of U251 cells stained with anti-ANO1 and anti-14-3-3γ antibodies. (d) Co-IP data show interactions between ANO1 and 14-3-3γ in U251 cells. Western blot images were cropped for clarification. Full blot images were included in supplementary information. (e) Representative images of Duolink PLA assay in U251 cells infected with adenovirus containing Sc shRNA and 14-3-3γ shRNA-1. Scale bar, 10 μm. (f) PLA signals were counted with Duolink Image tool software, and the average number of spots per cell is presented. Data are expressed as mean ± SEM; ***P < 0.001.

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