Figure 7: Inhibition of channel activity and trafficking of ANO1 in U251 cells decreases invasion and migration. | Scientific Reports

Figure 7: Inhibition of channel activity and trafficking of ANO1 in U251 cells decreases invasion and migration.

From: Suppression of 14-3-3γ-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells

Figure 7

(a) Representative images of collagen-coated transwell invasion assay in U251 cells infected with Sc shRNA, 14-3-3γ shRNA-1, and/ or ANO1 shRNA-1. (b) Normalized bar graphs showing data obtained from three independent experiments as in (a). Data are expressed as mean ± SEM; ***P < 0.001. (c) Representative images of wound-healing assay in U251 cells infected with Sc shRNA, 14-3-3γ shRNA-1, and/ or ANO1 shRNA-1. (d) Normalized bar graphs showing data obtained from three independent experiments as in (c). Data are expressed as mean ± SEM; ***P < 0.001. (e) Representative images (left panel) of collagen-coated transwell invasion assay in U251 cells treated with DMSO or A01 (10 μM for 18 h) Normalized bar graphs (right panel) showing data obtained from three independent experiments as in (left panel). Data are expressed as mean ± SEM; **P < 0.01. (f) Representative images (left panel) of wound-healing assay in U251 cells treated with DMSO or A01 (10 μM for 18 h) Normalized bar graphs (right panel) showing data obtained from three independent experiments as in (left panel). Data are expressed as mean ± SEM; ***P < 0.01.

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