Figure 3 | Scientific Reports

Figure 3

From: Abrogation of collagen-induced arthritis by a peptidyl arginine deiminase inhibitor is associated with modulation of T cell-mediated immune responses

Figure 3

Analysis of IgG subclass anti-collagen antibodies and serum cytokines indicates an induction of a Th2 response by treatment with BB-Cl-amidine.

(a–c) Pooled data from 2–3 independent CIA experiments are shown (n = 12–22 animals per group). (a) There was a significant rise in IgG1 anti–bovine collagen type II (bCII) antibodies in the 10 mg/kg BB-Cl-amidine treated group, with relatively little change in IgG2a anti-bCII. There was a fall in the IgG2a/IgG1 ratio with increasing BB-Cl-amidine treatment indicating induction of a Th2 response. AU, arbitrary units. (b) Increased serum levels of the Th2 cytokines IL-5 and IL-10. (c) There was no change in the pro-inflammatory cytokines IL-6, interferon-γ and TNF-α. (b,c) Each data point represents one animal. (a–c) Error bars represent ± SEM. One-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test relative to vehicle-treated animals. *P < 0.05; ***P < 0.001; ****P < 0.0001; ns, not significant.

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