Figure 4

BB-Cl-amidine restrains T cell numbers and proliferation in inguinal lymph nodes from mice with CIA.
(a–c) Data from CIA experiment is shown (n = 7 animals per group). (a) The total number of cells in the inguinal lymph nodes on day 10 was decreased with BB-Cl-amidine treatment. (b) The total number of CD4+ T cells in the inguinal lymph nodes of CIA mice is significantly lower in BB-Cl-amidine-treated mice. (c) BB-Cl-amidine decreases the percentage of proliferating CD4+ T cells (CD4+ BrdU+) in response to anti-CD3 antibody stimulation ex vivo. (a–c) Error bars represent ± SEM. One-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test relative to vehicle-treated animals. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.