Figure 6 | Scientific Reports

Figure 6

From: Abrogation of collagen-induced arthritis by a peptidyl arginine deiminase inhibitor is associated with modulation of T cell-mediated immune responses

Figure 6

BB-Cl-amidine inhibits the induction of Th1 and Th17 cells and reduces their numbers in vitro.

(a–d) Mouse CD4+ T cells were cultured in Th1, Th2 or Th17 differentiating conditions with BB-Cl-amidine or vehicle (DMSO) at 1 μM, 2.5 μM or 10 μM. After 5 days cell culture supernatants were investigated for cytokines, the cells were counted and analysed by flow cytometry. Culturing CD4+ T cells with BB-Cl-amidine decreases the amount of (a) IFN-γ, (b) IL-17A and (c) IL-4. (d) Culturing CD4+ T cells with BB-Cl-amidine decreases the total number of cells in Th1 conditions. (a–d) Shown is pooled data from 3 independent experiments with 3 biological repeats per experiment (n = 9 per group). Error bars represent ± SEM. One-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test relative to vehicle (control). *P < 0.05; **P < 0.01; ns, not significant.

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