Figure 2: ERRγ induces mitochondrial gene expression and content in db/db mice. | Scientific Reports

Figure 2: ERRγ induces mitochondrial gene expression and content in db/db mice.

From: Exercise-like effects by Estrogen-related receptor-gamma in muscle do not prevent insulin resistance in db/db mice

Figure 2

The following parameters were measured in 6 month-old male non-fasted db/+ (hatched bars), db/db (open bars) and db/db-ERRγ (black bars) mice. (A) Citrate synthase activity measured in biceps femoris muscles (N = 4). (B) Mitochondrial DNA content measured in biceps femoris muscles (N = 6–7). (C,D) Succinate dehydrogenase (SDH) and Nicotinamide Adenine Dinucleotide (NADH)-Tetrazolium Reductase (NADH-TR) staining in Tibialis Anterior (TA) muscles. (C) Representative images of the outer TA muscle. (D) Percentage of positive fibers in the outer TA stained positive for SDH (N = 5–6) and NADH-TR activity (N = 4–6). (E) Representative western blotting image of mitochondrial complex biomarker protein expression. *Indicates comparison to db/+ mice; †indicates db/db compared with db/db-ERRγ mice. (*/†p < 0.05, **/††p < 0.01, ***/†††p < 0.001, One-way ANOVA with Tukey’s post-hoc test).

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