Figure 1
From: MetaTrans: an open-source pipeline for metatranscriptomics
Flow diagram of the metatranscriptomic pipeline.
The raw paired-end reads were subjected to quality control and adjustment using the FastQC tool and Kraken pipeline (turquoise boxes). The rRNA/tRNA reads were then separated from the non-rRNA/tRNA reads using SortMeRNA software (green boxes), for taxonomic (clear blue boxes) and functional analyses (pink boxes), respectively. For the taxonomic analysis, the reads were mapped against the 16S rRNA Greengenes v13.5 database using SOAP2. For the functional analysis, the reads were subjected to the FragGeneScan to predict putative genes before being mapped against a functional database (MetaHIT-2014 or M5nr) also using the SOAP2 tool (see methods for details).
