Figure 3
From: Engineering the Campylobacter jejuni N-glycan to create an effective chicken vaccine
Chicken vaccinations and N-glycan-specific immune responses.
(A–D) Colonization levels of C. jejuni 81–176 are shown. Each data point represents a single bird, grey bars represent the median level of C. jejuni colonization for each group expressed as CFU per gram of cecal material (detection limit, 1 × 10*2 CFU). Birds in the negative control groups (PBS) were non-vaccinated, non-challenged; birds in the positive control groups (+ve) were non-vaccinated but challenged with C. jejuni 81–176. (A) Treatment groups included birds that were vaccinated by injecting either 100 μg or 5 μg of glycosylated ToxC-GT-His6 protein or non-glycosylated ToxC-GT-His6 protein (control) into the breast on days 7 and 21 before challenge with 10*2 or 10*6 CFU (as indicated) of C. jejuni 81–176 on day 28. (B) Treatment groups included birds that were vaccinated by injecting 5 μg of glycosylated ToxC-GT-His6 protein in the breast or in the leg on day 7 and 21 and in parallel, birds that were orally gavaged with 10*8 inactivated E. coli cells expressing the C. jejuni N-glycan on their surface before challenge with 10*2 CFU of C. jejuni 81–176 on day 28. (C). Birds in the treatment group were orally gavaged on day 7 and 21 with 10*8 live E. coli cells expressing the C. jejuni N-glycan on their surface before challenge with 10*2 CFU of C. jejuni 81–176 on day 28. (D) Birds in the treatment groups were orally gavaged on day 7 and 21 with either 10*8 live E. coli cells not expressing the N-glycan (control group) or 10*8 live E. coli cells expressing the C. jejuni N-glycan on their surface before challenge with 10*2 CFU of C. jejuni 81–176 on day 28. (E) N-glycan-specific antibody responses. ELISA results using a 1:10 dilution of chicken sera from bleeds taken prior to C. jejuni challenge (day 28). Each point represents the antibody response measured at OD405 for each individual chicken. Grey bars represent the median for each group. (A–E) Statistical differences between groups are indicated: ns, no statistically significant difference (p-value < 0.05); * and **indicate statistically significant differences with p-values > 0.05 and >0.005, respectively.
