Figure 3: PCA loading plot of 1H NMR spectra of 14-day-old Arabidopsis thaliana Col-0 whole above-ground tissue.

Compound identification of the most variable signals was performed by comparing corresponding 1H-13C HSQC signals with a database of authentic standards. The shown compounds are intermediates of the tricarboxylic acid cycle -TCA- (malate (orange), citrate (green), succinate (pink), and fumarate (purple)) or are involved in carbon:nitrogen balance (γ-aminobutyric acid (red) and glutamate (blue)).