Figure 1
Down-regulated expression of GCN5 in PDLSCs in inflammatory microenvironments.
(a) Western blot analysis showed the protein expression of GCN5 of freshly isolated H-PDLSCs and P-PDLSCs and GAPDH was used as the internal control. (b) Relative gene expression of GCN5 of 2, 4, 6 passage H-PDLSCs and P-PDLSCs was analyzed by qRT-PCR. Relative gene expression of GCN5 was determined based on the threshold cycle (CT) values. The expression levels of the target genes were normalized to that of the housekeeping gene GAPDH. (c) Western blot analysis showed the protein expression of GCN5 of 2, 4, 6 passage H-PDLSCs and P-PDLSCs and GAPDH was used as the internal control. (d) Western blot analysis showed the protein expression of GCN5 of 2 passage H-PDLSCs after either alone or in combination of LPS, TNF-α and IL-1β treatment. (e,f) Western blot analysis showed the protein expression of GCN5 of 4 and 6 passage H-PDLSCs, which were passed by 2 passage H-PDLSCs treated with either alone or in combination of LPS, TNF-α and IL-1β. GAPDH was used as the internal control. Data represent the means ± SD. *p < 0.05, **p < 0.01, (n = 3).
