Figure 2 | Scientific Reports

Figure 2

From: GCN5 modulates osteogenic differentiation of periodontal ligament stem cells through DKK1 acetylation in inflammatory microenvironment

Figure 2

Down-regulated GCN5 expression results in defective osteogenic differentiation of P-PDLSCs.

(a) Gene expression of Runx2, ALP and SP7 in H-PDLSCs and P-PDLSCs was measured by qRT-PCR after osteogenic induction for 14 days. (b) Protein expression of Runx2, ALP and SP7 in H-PDLSCs and P-PDLSCs was measured by western blot after osteogenic induction for 14 days. (c) H-PDLSCs and P-PDLSCs were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 28 days. Alizarin red was then extracted and measured for light absorbance at 562 nm. (d) Protein expression of GCN5 in single colony derived H-PDLSCs and P-PDLSCs was measured by western blot. (e,f) Single colony derived H-PDLSCs and P-PDLSCs were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 28 days. Alizarin red was then extracted and measured for light absorbance at 562 nm. Data represent the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, (n = 3).

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