Figure 4 | Scientific Reports

Figure 4

From: GCN5 modulates osteogenic differentiation of periodontal ligament stem cells through DKK1 acetylation in inflammatory microenvironment

Figure 4

GCN5 targets DKK1 to regulate osteogenic differentiation of PDLSCs.

(a) Western blot analysis showed expression of GCN5, Active-β-catenin, β-catenin, P-GSK3β, GSK3β of H-PDLSCs, H-PDLSCs/siGCN5 and P-PDLSCs. GAPDH was used as the internal control. (b) Gene expression of Wnt ligands and antagonists in H-PDLSCs and H-PDLSCs/siGCN5. Relative gene expression was determined based on the threshold cycle (CT) values. (c) Expression of DKK1, H3K9ac and H3K14ac of H-PDLSCs, H-PDLSCs/siGCN5 and P-PDLSCs was determined by western blot. (d) Protein expression of Runx2 in H-PDLSCs, H-PDLSCs/siGCN5 and H-PDLSCs/siGCN5+DKK1 was measured by western blot after osteogenic induction for 14 days. (e) H-PDLSCs, H-PDLSCs/siGCN5 and H-PDLSCs/siGCN5+DKK1 were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 28 days. Data represent the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, (n = 3).

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