Figure 6

Aspirin rescues the periodontitis of LPS-induced periodontitis rats through upregulating GCN5 expression.

(a) Alveolar bone loss determination of maxillary molars. Four sites for two molars (one site for each root of one tooth) were analyzed morphometrically. (b) Mean alveolar bone loss of two molars. (c) Gene expression of GCN5, IL-1β and TNF-α in periodontal tissue was determined by qRT-PCR. (d) GCN5 positive staining were showed in the periodontal ligament and its expression in the LPS group was significantly reduced compared to that of the control group. After aspirin (5 and 10 mg/ml) administration, the expression of GCN5 was greatly elevated and was comparable to that of the control group. Integrated intensity of GCN5 positive staining was measured by Image-Pro Plus 6.0. Scale bar: 100 μm, 200 μm. (e,f) Western blot showed expression of GCN5 and DKK1 of H-PDLSCs and P-PDLSCs with or without aspirin treatment, respectively. Data represent the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, (n = 3).