Figure 1: Dlg5 is required for follicle epithelial morphogenesis.

(A–D) Mutant clones of dlg5^EP2087 (A,B) and dlg5^KG748 (C,D) displayed morphological defects in follicle epithelia including cell flattening (A,C) and gap (B,D). Mutant clones were marked by the absence of GFP (green), nuclei were stained with DAPI (blue). (E) Quantification of morphological defects as displayed in (A–D), as well as from those rescued by dlg5 full length cDNA (number of clones indicated in parenthesis beside each genotype). (F–I) Knockdown of dlg5 caused similar epithelial defects as in (A–D). Control (act5C-GAL4,tub-GAL80^ts /+) egg chambers showed normal morphology of follicle cells (F). act5C-Gal4,tub-Gal80^ts/UAS-dlg5.RNAi flies were temperature shifted to 29 °C for 3 days and the egg chambers displayed cell flattening (G) and gap (H) phenotype. These phenotypes were rescued by UAS-Dlg5^RNAiRescue (I). Ovaries were stained with Crb (red) and DAPI (blue). (J) Quantification of morphological defects as displayed in (F–I) (number of egg chambers indicated in parentheses). (K) The domain organization of Drosophila Dlg5 protein. (L) Gene structure and mutant alleles of dlg5. Orange boxes represent the coding sequences, and grey boxes represent untranslated regions. The red lines indicate the sequences used in the VDRC RNAi lines. (M) The mRNA levels of dlg5 were reduced in dlg5 mutants as compared with the wild-type flies. Experiments were repeated independently three times with consistent results and three replicates were used per time point. Error bars represent s.e.m.; *P = 0.0263, **P = 0.0087, Student’s t test. Scale bars: 10 μm.