Figure 2: Dlg5 is specifically required for apical polarity and AJ. | Scientific Reports

Figure 2: Dlg5 is specifically required for apical polarity and AJ.

From: Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis

Figure 2

(A–L’) dlg5EP2087mutant clones in follicle epithelia displayed reduction of apical and AJ proteins including Crb (A–B’), Sdt (C,C’), PATJ (D,D’), aPKC (E–F’), Par6 (G,G’), Baz (H,H’), Arm (I,I’), E-cad (J,J’), N-cad (K–L’). The reduction of polarity proteins is stronger in clones with cell flattening (A,A’,E,E’,K,K’) than in clone with normal morphology (B,B’,F,F’,L,L’). (M–Q’) Basolateral and septate junction proteins Dlg (M,M’), Nrg (N,N’) and cytoskeletal components F-actin (O,O’), β-tubulin (P,P’), Sqh (Q,Q’) were not affected in dlg5EP2087 mutant clones. Mutant clones were marked by the loss of GFP or RFP (blue). (R) Quantification of the percentage of dlg5EP2087 or dlg5KG748 follicle cell clones that showed significant reduction of the polarity proteins (number of clones indicated below x-axis). The reduction of Crb, Sdt, PATJ, aPKC, Par6 and Ncad has high penetrance (>87% for dlg5EP2087) whereas the reduction of Baz, Arm and Ecad has intermediate penetrance (<63% for dlg5EP2087). Scale bar: 10 μm.

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