Figure 3: Double staining of apical proteins in dlg5 mutant clones.

(A–C”) In most cases, the degrees of reduction of Crb, aPKC and Par6 were comparable in dlg5^EP2087 mutant clones. The arrow in B” indicates a site where Crb was almost lost but Par6 was still present. (D–E”) In a minority of dlg5^EP2087 mutant clones, the reduction of Crb is distinctly stronger than that of aPKC. Arrows in (D”,E”) indicate the mutant clones where Crb was lost but aPKC was still present in a significant amount. Mutant clones were marked by the loss of GFP or RFP (blue). Scale bars: 10 μm.