Figure 4: Dlg5 localizes to the apical domain and AJ in early stage egg chambers and to the basolateral domain in stage 10 egg chambers.
(A) The confocal image of an ovariole shows that Dlg5-GFP colocalizes with Arm in germarium and early stage egg chambers. (B–B”) Dlg5-GFP (green) colocalized with Arm (red) at AJs in early stage egg chambers (indicated by arrows in B”). Dlg5-GFP spots also localized at apical domain between adjacent AJs (indicated by arrowheads in B” and correspond to spots in Fig. 6A–C’”). (C–D”) Dlg5-GFP spots localizes to the apical domain marked by aPKC (C–C”) or Crb (D–D”). Arrows indicate the Dlg5-GFP spots localized in the apical domain (compare to spots in Fig. 6A–C’”). (E–E”) In early stage egg chambers, Dlg5-GFP also localized to part of the lateral membrane marked by Dlg, but its colocalization with Dlg is restricted to regions adjacent to apical domain. (F–F”) In stage 9 egg chambers, Dlg5-GFP localized to both apical membrane and basolateral membrane. Note that the apical localization is stronger in follicle cells surrounding the nurse cells (NC) than that surrounding the oocyte. (G–I”) In stage 10 egg chambers, Dlg5-GFP localized to most of the basolateral domain marked by Dlg (red, I–I”), but did not localize to the AJ spots and apical membrane, marked by Arm (G–G”) and Crb (H–H”) respectively. Nuclei are stained with DAPI (blue). In all panels, the genotype is dlg5EP2087/dlg5EP2087; Ubi-Dlg5.GFP/TM3,Sb. Scale bars: 20 μm in A and 5 μm in others.
