Figure 7: Overexpression of Crb promotes basolateral localization of Dlg5.
(A–D”) In stage 7/8 egg chambers, basolateral spreading of Crb caused by Crb overexpression resulted in increased basolateral localization of Dlg5-GFP (B–B”), as compared to the wild type (A–A”). Dlg5-GFP localization was not affected in aPKC or Par6 overexpressing follicle epithelia (C–D”). (E–H”) In wild type stage 7/8 egg chambers, single confocal section (planar sectional view) at the apical and basolateral levels showed that Dlg5-GFP was enriched in the apical domain (E–E”) but not in the basolateral domain (F–F”). In Crb overexpressing follicle cells, Dlg5-GFP was enriched at both apical (G–G”) and basolateral membranes (H–H”). act5C-GAL4,tub-GAL80ts was used to drive expression in follicle cells. Scale bars: 10 μm.
