Figure 1: Expression profiles of Sortilin in immune cells and tissues. | Scientific Reports

Figure 1: Expression profiles of Sortilin in immune cells and tissues.

From: TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin

Figure 1

(a) Immunoblot analysis of sortilin in various immune cells. CD4+, CD8+ and B220+ cells were directly isolated from splenic cells by MACS. Macrophages were isolated from the peritoneal cavity, and other cells were derived from bone marrow cells. Whole cell lysates were separated by SDS-PAGE and sortilin expression was determined by immunoblot analysis. β-Actin was used as an internal control. CD4+, splenic CD4+ cells; CD8+, splenic CD8+ cells; B220+, splenic B220+ cells; pMØ, peritoneal macrophages; cDC, bone marrow-derived conventional DCs; pDC, bone marrow-derived plasmacytoid DCs; Mast, bone marrow-derived mast cells; Baso, bone marrow-derived basophils; Eo, bone marrow-derived eosinophils. Relative band intensities of sortilin compared to CD4+ are shown. (b) Sortilin expression in various tissues. Total RNA was isolated from mouse tissues and sortilin mRNA was quantified by qRT-PCR. GAPDH was used as an internal control for normalization. Data are mean ± SD (n = 3). Asterisks denote the statistical difference from brain. *p < 0.05 **p < 0.01.

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