Figure 2: Sortilin interacts with various cytokines.
From: TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin

The binding of cytokines to sortilin was measured by surface plasmon resonance analysis. Cytokine binding to immobilized recombinant sortilin was measured with a Biacore X instrument. Sensorgrams of surface plasmon resonance were obtained with various concentrations of recombinant IFN-α (a), IFN-γ (b), IL-6 (c), IL-10 (d), IL-12 (e), IL-17A (f) and IL-1β (g). BSA or GST was used as a negative control. All curves indicate the response after subtraction of non-specific binding, which was determined as the binding of analytes to a BSA-coated flow cell. The mean Kd values ± SEM (n = 3) are shown.