Figure 4: TLR signals lead to posttranscriptional degradation of sortilin.
From: TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin

(a,b) The time course of sortilin degradation following CpG-A (3 μM) stimulation was determined by immunoblotting (a) and qRT-PCR (b) in pDCs. The asterisk represents a nonspecific band. (c,d) The time course of sortilin degradation following LPS (0.1 μg/ml) or CpG-B (3 μM) stimulation was determined by immunoblotting (c) and qRT-PCR (d) in macrophages. Data are mean ± SD (n = 3). (e) Quantification of sortilin mRNA after stimulation with various TLR ligands in macrophages. Macrophages were stimulated with poly I:C (10 μg/ml), LPS (1 μg/ml), imiquimod (10 μg/ml) and CpG-B (3 μM) for 6 h, followed by quantification of sortilin mRNA by qRT-PCR. Data are mean ± SD (n = 3). (f) Posttranscriptional degradation of sortilin mRNA. Sortilin mRNA was quantified by qRT-PCR after CpG-B stimulation (1 μM) of cells in medium containing 1 μg/ml ActD. Linear regression analyses were performed with GraphPad Prism software to determine the half-life of sortilin mRNA. Data are mean ± SD (n = 3). *p < 0.05.