Figure 1

Extracellular CIRP induces vascular leakage and causes lung injury.

The C57BL/6 mice were intravenously injected with vehicle (normal saline) or recombinant murine CIRP (rmCIRP, 5 mg/kg body weight) and lungs were collected for various analysis 5 h later. (A) Representative images of the lungs stained with Evans blue dye (EBD) post-vehicle or rmCIRP injections. (B) Colorimetric analysis of EBD extracted from stained lungs. Bars represent mean ± SEM (n = 5 per group). *P < 0.05; Student’s t-test. Representative images of H&E stained lung sections from (C) vehicle or (D) rmCIRP injected mice at original magnification ×400. The qPCR analysis of (E) cytokines and (F) endothelial cell adhesion molecules in the lungs of vehicle or rmCIRP injected mice. Bars represent mean ± SEM (n = 3 per group). *P < 0.05; Student’s t-test.