Figure 6: Identification of the protein–protein interaction between TFV protein containing putative L-domain and corresponding host protein using immunoprecipitation (IP). | Scientific Reports

Figure 6: Identification of the protein–protein interaction between TFV protein containing putative L-domain and corresponding host protein using immunoprecipitation (IP).

From: Budding of Tiger Frog Virus (an Iridovirus) from HepG2 Cells via Three Ways Recruits the ESCRT Pathway

Figure 6

(A) IP of VP-031L and Alix using anti-Alix antibody. Hela cells were transfected with pVP-031L-myc, pVP-031L-L90A-myc, or an empty vector pCMV-myc. Cells were processed for IP as described under “Materials and Methods”. Anti-myc antibody was used as the primary antibody for IP. (B) IP of VP-065L and Tsg101 using anti-myc antibody and anti-Tsg101 antibody. Hela cells were transfected with pVP-065L-myc, pVP-065L-A342L-myc, or an empty vector pCMV-myc. Cells were processed for IP as described under “Materials and Methods”. Anti-Tsg101 antibody and anti-myc antibody were used as the primary antibodies for IP. (C) IP of VP-093L and Nedd4 using anti-Nedd4 antibody. Hela cells were transfected with pVP-0931L-myc, pVP-093L-P115A-myc, or an empty vector pCMV-myc. Cells were processed for IP as described under “Materials and Methods”. Anti-myc antibody was used as the primary antibody for IP.

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