Figure 4: Effects of alternating electric field on lung cancer cell aggregates.
From: Engineering a 3D microfluidic culture platform for tumor-treating field application
Normalized dispersion (Δ/Δ0) (A) and proliferation rate (B) of A549 lung cancer cell aggregates after 24, 48, and 72 h of stimulation at a frequency of 200 kHz and 1.1 V/cm intensity. (C) Representative confocal images of A549 aggregates at 0, 24, 48, and 72 h under DMSO treatment (positive control), under electric field stimulation and without stimulation (negative control). Blue = cell nuclei and red = dead cells. Scale bars = 50 μm. (D) Percentage of Ki-67+ lung cancer cells (A549) and endothelial cells (HUVEC) stimulated at a frequency of 200 kHz and intensity of 1.1 V/cm. Representative images with Ki-67 expression (in red, lower panel). Unpaired t-test, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s = not significant. Scale bars = 100 μm.
